It uses an iTAGTM dye that has two keyproperties: an aromatic nature, which provides the orange colorresponsible for spectral absorbance readings at 483 nm, and anacid group (Urh, 2008; Amamcharla and Metzger, 2010; Mooreet al., 2010) that binds to the cationic groups of basic amino acids(Histidine, Arginine, and Lysine) and the amino terminal groups ofproteins. As these specific amino acids have a high pKa (Urh, 2008),