The fraction eluted with MeOH (1.95 g) afforded atisonium chloride 78 (1.3 g) after crystallization from EtOAc-MeOH (9:1). The acid aqueous phase was neutralized to pH 7 and extracted with CHCl3 to give a crude material (9.5 g).
Thefraction eluted with MeOH (1.95 g) afforded atisonium chloride78 (1.3 g) after crystallization from EtOAc-MeOH (9:1). Theacid aqueous phase was neutralized to pH 7 and extracted withCHCl3 to give a crude material (9.5 g).