Agriculture, Abbasia, Egypt, were used as semen donors.
Ejaculates were collected using a bovine adapted artificial
vagina at weekly intervals for 3 weeks. Semen samples
were initially evaluated for subjective sperm motility and
sperm concentration. Ejaculates fulfilling minimum
standard of sperm motility (70%) and sperm morphology
pooled in order to have sufficient semen for a replicate
and to eliminate the bull effect. The semen was given a
holding time for 10 minutes at 37°C in the water bath
before dilution.