Other reasons for later occurrence of infections could be
inaccurate work (non-sterile forceps or scalpels; working
surface not sterilised with 96 % alcohol; not sufficiently
sterilised nutrient media; etc.) (Pierik 1998). It is well known,
that the growth and proliferation of roses depend strongly on
cultivar (Kane 2000). Horn et al. (1988) reported that nodal
explants showed better results than shoot tips and that the
subcultured shoots with long internodes were more suitable
than subcultures from compact shoots with short internodes
(in means of growth, number, length and fresh weight of
newly obtained shoots).