Fig. 1.HPP-induced germination ofC. perfringensspores. Spores were treated at 50
C
for 7 min with: 100 MPa (circles); 150 MPa (squares); 200 MPa (triangles); and
germination was assessed by measuring decrease in OD600 after incubating these
pressure-treated spores at 40
C for 60 min in 25 mM sodium phosphate buffer as
described inMaterials and methods.