After attachment of the microorganism, each SAM was placed in sterile
stomacher bag containing 100 mL of 20 g/kg LA plus 10 g/kg SDS or
20 g/kg AA plus 10 g/kg SDS and gently massaged for 3 min. Each
treated SAM was placed into a sterile stomacher bag containing
10 mL of peptone water (1 g/kg)