Five commercially available primers were shown to be useful for discrimination of F.elongatus, O.streptocoelium and P.epiclitum. The phylogenetic tree constructed
with PAUP program from 5 HAT-RAPD output data grouped P.epiclitum and O.streptocoelium (subfamily Paramphistominae) together, and placed F.elongatus (subfamily Gastrothylaxinae) in another group.