Samples
(1 mL) for determination of total bacteria were fixed with glutaraldehyde
(0.25% final concentration) and frozen in Eppendorfs
at
−80 ◦C until analyses. After thawing, the samples were serial
diluted to 1:5, 1:10 and 1:50, stained with SYBR Green and analyzed
according to Marie et al. (2005) using a FACS Caliber flow cytometer
(Becton Dickson).