The greatest advantage of selfcalibration is that the affects of environmental conditions including temperature, humidity, pressure, or etc. can be minimized to a large extent. In this situation, no consideration of different conditions is necessary to be included and systematic errors can be avoided, the semiquantitative assay can also be achieved rapidly. Thus, the paper-based sensor provides a rapid, point-of-care testing of glucose in resource limited areas and developing countries. However, Y-shaped strip should be chosen for detecting single component.