The substance that is dissolved, all elements contained in a buffer solution to a crisis that is appropriate per reaction. Reaction in vitro To begin adding high temperatures until the DNA cables. A DNA template. Double split into DNA A single string, and then reduce the temperature down to a match between DNA template and primers with a hydrogen bond, and then adjust the temperature to the proper operation of the enzyme DNA polymerase to DNA replication DNA line by line template, then add it to a higher temperature to extract DNA double in round 1, the reaction has been carried out activity, such as this duplicates many of the original period of the DNA molecule that it would have to be a lot of.