under the following reaction conditions: 94 C for 2 min, followed 27 cycle of 94 C for 30 s, 55 C for 30 s, 72 C for 1 min, and the product was inserted into the pGEM-T cloning vector
under the following reaction conditions:94 C for 2 min, followed 27 cycle of 94 C for 30 s,55 C for 30 s, 72 C for 1 min, and the product wasinserted into the pGEM-T cloning vector