Novel mungbean germplasm derived from induced mutation
Variegated leaf Variegated mutant was obtained from Gamma-rays irradiation at a rate of 500 gray. All F1 plants from the cross between the variegated mutant and the normal leaf parent showed normal green leaves without reciprocal, while the F2 plants segregated well in a 3 : 1 ratio. The number of F3 lines showing all green plants, segregating, all variegated plants fitted well with the 1 : 2 : 1 ratio. Thus it can be inferred that the variegated leaf character is controlled by a single recessive nuclear-encoded gene. We propose var1 as the gene symbol [10].
Multiple leaflet Two new multiple leaflet mungbean mutants were also obtained from Gamma-rays irradiation. A mutant with small pentafoliate was crossed with a large heptafoliate mutant to study the inheritance at Kasetsart University, Kamphaeng Saen Campus. AFLP markers were also screened
to make a partial linkage map around the genes controlling multifoliate leaflets. The number of F2 plants was tested against a 3 , 1 ratio for segregation in a single locus and 9 : 3 : 3 : 1 for 2 independent loci using the Chisquare goodness of fit test. The heterogeneity among the F2 families was also tested accordingly. Crossing between 7 large leaflet (L-7) and 5 small leaflet (S-5) mungbean mutants resulted in the normal-trifoliate (N) F1. The F2 can be classified into number of leaflet per leaf and leaflet size with large-trifoliate ( N 1 -N 2 ), small-pentafoliate ( N 1 -n 2 n 2), large-heptafoliate ( n 1 n 1 N 2 ), and small-heptafoliate ( n 1 n 1 n 2 n 2) at the dihybrid ratio of 9 : 3 : 3 : 1. The finding is thus evident that leaflet number was controlled by n 1 locus and leaflet size was controlled by n2 locus of genes, respectively. However, all three AFLP markers associated with leaflet number in this study corresponded to n1 locus only. The n 2 locus can have a pleiotropic effect upon the leaflet size such that the N 2 allele controls large leaflet size as well. Another hypothesis is that the n 2 locus might be closely linked with the s locus so that there was no progenies with large pentafoliate leaflet (hypothetically carrying N 1 - n 2 n 2 S -genotype). [10]
Novel mungbean germplasm derived from induced mutation Variegated leaf Variegated mutant was obtained from Gamma-rays irradiation at a rate of 500 gray. All F1 plants from the cross between the variegated mutant and the normal leaf parent showed normal green leaves without reciprocal, while the F2 plants segregated well in a 3 : 1 ratio. The number of F3 lines showing all green plants, segregating, all variegated plants fitted well with the 1 : 2 : 1 ratio. Thus it can be inferred that the variegated leaf character is controlled by a single recessive nuclear-encoded gene. We propose var1 as the gene symbol [10]. Multiple leaflet Two new multiple leaflet mungbean mutants were also obtained from Gamma-rays irradiation. A mutant with small pentafoliate was crossed with a large heptafoliate mutant to study the inheritance at Kasetsart University, Kamphaeng Saen Campus. AFLP markers were also screened to make a partial linkage map around the genes controlling multifoliate leaflets. The number of F2 plants was tested against a 3 , 1 ratio for segregation in a single locus and 9 : 3 : 3 : 1 for 2 independent loci using the Chisquare goodness of fit test. The heterogeneity among the F2 families was also tested accordingly. Crossing between 7 large leaflet (L-7) and 5 small leaflet (S-5) mungbean mutants resulted in the normal-trifoliate (N) F1. The F2 can be classified into number of leaflet per leaf and leaflet size with large-trifoliate ( N 1 -N 2 ), small-pentafoliate ( N 1 -n 2 n 2), large-heptafoliate ( n 1 n 1 N 2 ), and small-heptafoliate ( n 1 n 1 n 2 n 2) at the dihybrid ratio of 9 : 3 : 3 : 1. The finding is thus evident that leaflet number was controlled by n 1 locus and leaflet size was controlled by n2 locus of genes, respectively. However, all three AFLP markers associated with leaflet number in this study corresponded to n1 locus only. The n 2 locus can have a pleiotropic effect upon the leaflet size such that the N 2 allele controls large leaflet size as well. Another hypothesis is that the n 2 locus might be closely linked with the s locus so that there was no progenies with large pentafoliate leaflet (hypothetically carrying N 1 - n 2 n 2 S -genotype). [10]
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