by a clear and compact cumulus cell mass were cultured in 500 µl of mPOM containing 10 IU/ml eCG, 10 IU/ml hCG and 1 mM dibutyryl cAMP in an atmosphere of 5% CO2 in air at 39 C for 20 h. Then the culture was continued in a fresh mPOM medium without the above supplements for another 24 h period [26].