The polyphenols in cocoa beans undergo oxidation reaction which is both non-enzymatic and enzymatic through the action of polyphenol oxidase. Hansen et al. [33] reported this enzyme is strongly inactivated during the first day of fermentation, remaining only 50 and 6% of enzyme activity after 1 and 2 days, respectively. Forsyth [11] reported loss of polyphenols caused by diffusion into the fermentation sweatings and confirmed by the microscopic studies carried out by de Brito et al.