The leaves were washed with distilled water and were
shade dried at room temperature. The dried parts are
powdered with the help of an electric blender. The dried
powder was subjected to extracts with Aqueous, petroleum
ether and chloroform for 8 h in a Soxhlet apparatus (Sigma,
Mumbai). The plant extracts were evaporated to dryness in
rotary vacuum evaporator. The residue was then made into
a 1% stock solution with acetone. The stock solutions for
various test concentrations were prepared.