Biodegradation has been proposed as an effective approach to remove pyrene, however, the information
regarding cellular responses to pyrene treatment is limited thus far. In this study, the biodegradation and
biosorption of pyrene by Brevibacillus brevis, along with cellular responses caused by pollutant were
investigated by means of flow cytometry assay and scanning electron microscopy. The experimental
results showed that pyrene was initially adsorbed by B. brevis and subsequently transported and intracellularly
degraded. During this process, pyrene removal was primarily dependent on biodegradation.
Cell invagination and cell surface corrugation occurred due to pyrene exposure. Nevertheless, cell regrowth
after 96 h treatment was observed, and the proportion of necrotic cell was only 2.8% after pyrene
exposure for 120 h, confirming that B. brevis could utilize pyrene as a sole carbon source for growth. The
removal and biodegradation amount of pyrene (1 mg/L) at 168 h were 0.75 and 0.69 mg/L, respectively,
and the biosorption amount by inactivated cells was 0.41 mg/L at this time.
Biodegradation has been proposed as an effective approach to remove pyrene, however, the informationregarding cellular responses to pyrene treatment is limited thus far. In this study, the biodegradation andbiosorption of pyrene by Brevibacillus brevis, along with cellular responses caused by pollutant wereinvestigated by means of flow cytometry assay and scanning electron microscopy. The experimentalresults showed that pyrene was initially adsorbed by B. brevis and subsequently transported and intracellularlydegraded. During this process, pyrene removal was primarily dependent on biodegradation.Cell invagination and cell surface corrugation occurred due to pyrene exposure. Nevertheless, cell regrowthafter 96 h treatment was observed, and the proportion of necrotic cell was only 2.8% after pyreneexposure for 120 h, confirming that B. brevis could utilize pyrene as a sole carbon source for growth. Theremoval and biodegradation amount of pyrene (1 mg/L) at 168 h were 0.75 and 0.69 mg/L, respectively,and the biosorption amount by inactivated cells was 0.41 mg/L at this time.
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