DPPH
(Diphenyl picrylic hydrazine) reagent was added and incubated
for 45 min in dark. Methanol was taken as blank and 3 ml
methanol with 0.5 ml DPPH reagent was taken as control. The
absorbance was measured using spectrophotometer (Shimadzu
1609, Tokyo, Japan) at 517 nm. The antioxidant activity
was calculated using the following formula: