This study aims to investigate the potential of using ultrasound-assisted tapping technology to increase rubber yield from the Para rubber tree H. brasiliensis. The influence of ultrasound treatment time (0, 1–4 min, respectively) on latex yield, dry rubber content and relative rubber yield was evaluated and compared with untreated trees. The impact of sonication on the biochemical compositions of the latex was also examined as they provide very useful information on the status of the laticiferous system.