Legume seeds (cowpea, adzuki bean or mung bean) with
approximately two-day old Callosobruchus eggs were placed in
solid watch glasses containing colchicine solution (0.1% colchicine
solution in buffered insect saline (0.75% sodium chloride made up
in distilled water buffered to pH 6.8 with Sörensen’s phosphate
buffer)). Under a binocular microscope the eggs were carefully
dislodged from the seeds with fine pins