In order to answer our questions with a GSMM we need a model that:
contains a comprehensive representation of P. putida metabolism
is capable of predicting growth rates in various environments
includes all reactions that are important for the BananaGuard system
For this reason a comparison was made between various available GSMMs for P. putida. We took a in-depth look in two models that were up-to-date, published and verified: iJP962 and iJN746[15,16]. For the necessary work to model the metabolic stress in P. putida, the best starting model needs to be chosen which has the most potential for accurately modeling the metabolic stress of our system.