T-RFLP analysis. Bacterial population structures in different particle size fractions of a long-term field experiment receiving
different fertilizer amendments were determined by T-RFLP analysis (Fig. 1). Replicates showed a certain degree
of variation; however, they grouped together when all profiles were compared. A total of 55 T-RFs were detected, and because some fragments were only present in individual replicates,the total number of T-RFs found in the representative
sample profiles decreased to 43. The number of T-RFs in individual sample profiles ranged from 7 (fallow and peat, sand
fraction) to 26 (AM, clay fraction) (Table 1). In all treatments,the bacterial diversity was lowest in the sand fractions and
highest in the clay fractions. The only exception was the treatment with peat as an organic amendment, in which the highest number of T-RFs was found in the silt fraction (Table 1).Treatments involving amendments of GM and AM showed
higher diversities, particularly in clay size fractions, than treatments without organic amendments. Additionally, the sand
fraction of the GM treatment exhibited an unusually high diversity compared to the sand fractions of other treatments.
Some fragments present in several treatments showed far greater fluorescence signals in samples of the GM treatment.
T-RFLP analysis. Bacterial population structures in different particle size fractions of a long-term field experiment receivingdifferent fertilizer amendments were determined by T-RFLP analysis (Fig. 1). Replicates showed a certain degreeof variation; however, they grouped together when all profiles were compared. A total of 55 T-RFs were detected, and because some fragments were only present in individual replicates,the total number of T-RFs found in the representativesample profiles decreased to 43. The number of T-RFs in individual sample profiles ranged from 7 (fallow and peat, sandfraction) to 26 (AM, clay fraction) (Table 1). In all treatments,the bacterial diversity was lowest in the sand fractions andhighest in the clay fractions. The only exception was the treatment with peat as an organic amendment, in which the highest number of T-RFs was found in the silt fraction (Table 1).Treatments involving amendments of GM and AM showedhigher diversities, particularly in clay size fractions, than treatments without organic amendments. Additionally, the sandfraction of the GM treatment exhibited an unusually high diversity compared to the sand fractions of other treatments.Some fragments present in several treatments showed far greater fluorescence signals in samples of the GM treatment.
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