2.3. Botanical fungicide formulation
The botanical fungicide Damet 50 EC is prepared in Plant
Pathology laboratory, TNAU, Coimbatore, Tamil Nadu. The method
of preparation is as follows. Two kg of D. metel leaves were washed
and ground with methanol (1:2) and filtered several times through
cheese cloth and the supernatant solution was collected. The clear
solution was condensed in vacuum and reduced to half the volume.
Fifty EC formulation was developed using this condensed, partially
purified extract (100% concentration) by adding the recommended
quantities of emulsifying agent (Unitox 30x and 60y), stabilizing
agent (Epichlorohydrin) and solvent (Cyclohexanone) and named
as Damet 50 EC.