2.4. Polymer-enhanced enzymatic microalgal cell disruption
Cell disruption andlipid extraction were conducted in capped 15-mL
test tubes witha 5 mL working volume. Initially, 1 mL prepared algal cell
suspension was charged into each test tube, followed by the addition of
500
μ
L of prepared polymer stock solution and/or 500
μ
L of enzyme
stock depending on the treatment (
Table 1
). Blank indicates the control
measurement without enzyme or polymer solutions (microalgal bio-
mass and DI water only). For the enzyme control treatment, algal cell
suspensions were mixed with enzyme solutions and DI water without
any polymer. For combined enzyme and polymer treatments, three