3.2. Determination of the amino acid sequences and molecular masses of the peptides
Using a Protein/Peptide Sequencer and ESI-MS, the amino acid sequences of BCP-A and BCP-B were determined to be Trp- Pro-Pro (WPP, BCP-A) and Gln-Pro (QP, BCP-B), respectively (Fig. 4), with MWs of 398.44 Da ([M + H]+ 399.27 Da) and 243.23 Da ([M + H]+ 244.14 Da), which agreed with the theoretical masses of 389.46 Da and 243.26 Da, respectively.