at about 80°C. It was removed and cooled in the dessicator
and later weighed, W3. The flask containing lipid, boiling
chips and solvent was heated in a water bath to evaporate
the solvent and later dried and weighed WB.
Calculations:
% Lipid from thimble = W3 – W1 x 100
W2 – W1
%Lipid from oil in the flask = WB – WA x 100
W2 – W1
Determination of Carbohydrate
Carbohydrate content was determined by difference,
deducting the sum of the moisture content, ash content,
protein content, lipid content crude fiber content from one
hundred.
Determination of Specific Mineral Content, Using
Atomic Absorption Spectroscopy (AAS).
The mineral content was determined using the method
described by Suzanne Nielson
[10].
Exactly 6g of the food
was ashed by burning it in a muffle furnace at temperature
of about 6500
C. The resulting ash was dissolved in a 2%
HCl. The solution was then aspirated into the instrument
where it was heated to vapourized and atomized the
minerals.
at about 80°C. It was removed and cooled in the dessicator
and later weighed, W3. The flask containing lipid, boiling
chips and solvent was heated in a water bath to evaporate
the solvent and later dried and weighed WB.
Calculations:
% Lipid from thimble = W3 – W1 x 100
W2 – W1
%Lipid from oil in the flask = WB – WA x 100
W2 – W1
Determination of Carbohydrate
Carbohydrate content was determined by difference,
deducting the sum of the moisture content, ash content,
protein content, lipid content crude fiber content from one
hundred.
Determination of Specific Mineral Content, Using
Atomic Absorption Spectroscopy (AAS).
The mineral content was determined using the method
described by Suzanne Nielson
[10].
Exactly 6g of the food
was ashed by burning it in a muffle furnace at temperature
of about 6500
C. The resulting ash was dissolved in a 2%
HCl. The solution was then aspirated into the instrument
where it was heated to vapourized and atomized the
minerals.
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