suspend 37 grams in 1000 ml distilled water weat to boring to dissolves the medium completely sterilize by autoclaving at 15 ibs presure 121 for 15 munutes Avoid overheating cool to 45-50 and shake the medium in order to oxidize the methlene blue 1 c restore it is blue colour and to suspend the precipitate wich is and essential part of the medium