*The presence of GA was detected by TLC analysis (p.
37) and sign. (+) denotes its formation, while sign. (-)
means that the microorganism fails to produce GA.
Thin layer chromatography (TLC) was performed on silica
gel plates, (Fluka, silica gel 60F-254, layer thickness 0.2
mm) using the following solvent systems:
A. Chloroform – petroleum ether – acetic acid (6:6:1, v/v).
B. Acetic acid – n-butanol – 1,2-dichloroethane – water)