The new study used a simulated gut model and further tests in a model colon to assess the survival of the probiotic strain Lactobacillus casei 01 and its subsequent influence on on the make-up of the microbiota. Led by Tanachai Pankasemsuk from Chiang Mai University in Thailand, the team noted that previous studies investigating the potential of alginate and hi-maize combinations found that total short-chain fatty acids produced by the gut microbiome increased by nearly 17% while levels of lactobacilli and bifidobacteria in the colon increased by 2 and 1 log colony forming units (CFU) per mL, respectively.“To confirm the previous work, an alginate and a high-maize starch were used to entrap L. casei 01, subsequently, the influence of this composite material on the cell survivability in a gut model including its metabolites were investigated,” wrote the team.Pankasemsuk and colleagues reported that the alginate beads incorporating 1% hi-maize starch enabled optimal levels of viable cells in both gastric and bile fluids.“In general, greater survival of entrapped L. casei 01 than the free cells upon incubation in the simulated gastric and bile fluids was achieved,” revealed the team.“In the colon experiment, coated L. casei 01 had great influence on the augmentation of colon lactobacilli, whereas SCFA such as acetate, propionate and butyrate were the major synthesized products of colon microbiome
The new study used a simulated gut model and further tests in a model colon to assess the survival of the probiotic strain Lactobacillus casei 01 and its subsequent influence on on the make-up of the microbiota. Led by Tanachai Pankasemsuk from Chiang Mai University in Thailand, the team noted that previous studies investigating the potential of alginate and hi-maize combinations found that total short-chain fatty acids produced by the gut microbiome increased by nearly 17% while levels of lactobacilli and bifidobacteria in the colon increased by 2 and 1 log colony forming units (CFU) per mL, respectively.“To confirm the previous work, an alginate and a high-maize starch were used to entrap L. casei 01, subsequently, the influence of this composite material on the cell survivability in a gut model including its metabolites were investigated,” wrote the team.Pankasemsuk and colleagues reported that the alginate beads incorporating 1% hi-maize starch enabled optimal levels of viable cells in both gastric and bile fluids.“In general, greater survival of entrapped L. casei 01 than the free cells upon incubation in the simulated gastric and bile fluids was achieved,” revealed the team.“In the colon experiment, coated L. casei 01 had great influence on the augmentation of colon lactobacilli, whereas SCFA such as acetate, propionate and butyrate were the major synthesized products of colon microbiome
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