3.4. Effects of various propolis samples on ABTS radical cation
Most of the EEP samples showed ABTS radical cationscavenging activity (Fig. 4). The ABTS radical cation
decolorization assay is a spectrophotometric method widely used for the assessment of antioxidant activity of
various substances (Erel, 2004). The ABTSÆ+ is generated by the oxidation of ABTS with potassium persulfate and
is reduced in the presence of such a hydrogen-donating antioxidant. EEP samples were evaluated at a final concentration
of 500 lg/ml for the assay. Trolox and VE were compared at 50 lg/ml under the same conditions.