A filter-sterilized stock solution of each agent was added
to the autoclaved medium. Plastic Petri dishes (90 20 mm) were filled with 30 ml of the
medium, and 0.1 g FWof the calli per dish were placed. For examining callus growth, the
calli on C media were cultured in the dark, while for examining somatic embryo formation,
those on E media were incubated under continuous illumination.