Finally, in the second part of the experiment, earthworm health
after a period of depuration was assessed by measuring various
parameters and using advanced techniques, such as apoptosis
frequencies (TUNEL test), Ag and Co contents in earthworm body
tissues (ICP-OES), scanning electron microscopy coupled with Xray
probe for microanalysis (ESEM-EDS), and X-ray computed
microtomography (X-ray micro-CT).