Samples were stirred with 20 ml of chloroform/methanol mixture (1:1 v/v) at room temperature for 2 – 3 h, were followed by centrifugation at 2000xg for 5 min at room temperature
to separate the aqueous part and organic lower phase part. The lower phase containing lipid was recovered by Pasteur pipette, and evaporated under reduced pressure for 10 min. The dry lipid was weighed.