Phosphate buffered saline (PBS), collection medium for PFs (HEPESbuffered tissue culture medium 199 supplemented with 0.5% bovine
serum albumin (BSA), 50 g/mL gentamicin sulphate, 0.23 mM of sodium
pyruvate, 2 mM l-Glutamine and 25 IU/mL heparin), handling medium
(collection medium without heparin), stock solutions ofEGF and FSH were
all prepared as described by Tamilmani et al. (2005).
Phosphate buffered saline (PBS), collection medium for PFs (HEPESbuffered tissue culture medium 199 supplemented with 0.5% bovineserum albumin (BSA), 50 g/mL gentamicin sulphate, 0.23 mM of sodiumpyruvate, 2 mM l-Glutamine and 25 IU/mL heparin), handling medium(collection medium without heparin), stock solutions ofEGF and FSH wereall prepared as described by Tamilmani et al. (2005).
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