3.4. Effect of different citrus peel extracts on the expression of COX-2
and iNOS
To explore the inhibitory effect of citrus peel extracts on the
expression of COX-2 and iNOS, we determined the protein and
mRNA levels by immunoblotting and RT-PCR analysis, respectively.
Fig. 2 shows that RAW 264.7 macrophages did not express detectable
levels of COX-2 and iNOS proteins when incubated in culture
medium without LPS but, when incubated in the medium containing
LPS (1 lg/ml), cells markedly increased both COX-2 and iNOS