To analyze the expression profile of CsCath L during
infection, C. striatus was injected with A. invadans
and A. hydrophila and the liver was analyzed
by real time PCR (Fig. 6b,c). In A. invadans infected
tissue, the highest level of CsCath L mRNA transcripts
was observed at 24 h post-injection (Fig. 6b).
Significant differences (P < 0.05) were found in expression
at 3, 6, 12 and 24 h post-injection between
A. invadans injected and the PBS injected control
groups. In A. hydrophila injected groups, the level of CsCath L mRNA transcripts sharply increased until
24 h post-injection and the expression was decreased
at 48 h post-injection, almost near to the basal level
(Fig. 6c). The expression was significantly different (P
< 0.05) in all the post-injection time points between
A. hydrophila challenged and the PBS injected control
groups.