The aim of this work was the design and the structural and functional characterization of different liposomal formulations
based on SPC with bioactive compounds (omega-3, omega-6, VE) and VC for developing a functional food in pasteurized chocolate milk. Essential fatty acids like linolenic acid (x-3) and linoleic acid (x-6) are contained in SPC and in the formulations, the stearic acid (SA) and the calcium stearate (CaS) were incorporated for stabilize the lipid bilayer by increasing rigidity. Besides, addition of CaS incorporated a mineral (calcium) that increases the nutritional value. Oxidative stability was determined with the thiobarbituric acid method. The size and the shape were analyzed by light scattering
and light microscopy, respectively. And the packing of membrane was studied with two probes (merocyanine 540 and
Laurdan). All studies were performed with or without vitamins and before and after pasteurization in order to have information about the effect of the heat treatment. Pasteurization was applied for preserve the liposomes. Also, encapsulation efficiency of FA was determined after 72 h of dialysis in pasteurized formulations.