Total mRNA derived from
mycelia cultivated on Avicel cellulose as a sole carbon source was
reverse-transcribed, and quantitative real-time PCR analysis was
performed using the cDNA as a template and a gene-specific primer
set. In general, the expression level of all the b-glucosidase genes
was relatively low compared to the cbh1 and egl1 genes (Fig. 2A and
B). The expression level of cel3b, which encodes Cel3B, was
extremely low compared to the cellulase genes, but a low level of
expression could nevertheless be observed (Fig. 2B).