Fig. 4. Re-establishment of ‘active copulatory readiness’ in Chorthippus biguttulusfemales after copulation. (a and b) Completed copulationswere verified by the analysis of female
bursae for sperm content on the sixth day following copulation. Photographs of histological sections through the bursa copulatrix show its lumen (lu)with surrounding epithel
cells. While lumina of bursae copulatrix of virgin control females were unlabelled (a) all experimental females contained strong anti-tubulin immunoreactivity (s) that was
indicative of sperm (b). (c) Proportion of females (N = 24) that performed male song-stimulated stridulation immediately after mating and on 5 subsequent days. (d) Relative
duration of male song-stimulated sound production in females (N = 8) before mating, immediately after mating and on 5 subsequent days. The females’ responsiveness was
significantly reduced until the second day after mating (Friedman test, Wilcoxon–Wilcox test for multiple comparisons of matched samples). (*) Significant with p < 0.05.