In this study, Box-Behnken response surface experimental
design (BBD) with three factors at three levels was
used to optimize and investigate the influence of process
variables such as extraction temperature (40 - 60
˚C), time (20–100 min) and solid–liquid ratio (1:5–
1:15 g/ml) on the TMAC and TCS of the extracts.
Process variables and their ranges were determined
based on the single factor experimental analysis. After
selection of process (independent) variables and their
ranges, experiments were established based on a BBD
and the complete design consists of 15 experiments with
three centre points (used to estimate the experimental
error). The total number of experiments was calculated
from the following equation (Prakash Maran et al.
2013a).