Pre-treatment Label Description Conditions Pre-treatment Label Description Conditions
i
Heat shock
WB 90°C/
20 min
WB + G + n @90°C for
20min
iii
Chemical
addition
pH3
G + n + HCl
addition of HCL
25% for pH3
during 24hr
MW 2.5min
@900W
MW + G + n
2.5 min under
900W BES 5mM G +n + BES 5 mM
MW 5 min
@900W
5 min under
900W BES 7mM 7 mM
MW 7 min
@900W
7 min under
800W BES 8 mM 8 mM
MW 10min
@900W
10 min under
800W BES 10 mM 10 mM
ii
Combination:
heat shock
and chemical
addition
WB 90°C +
pH4
WB + HCl +
G + n
@ 90°C for
20min + pH4
during 17hr
CHCL3
0.05%
G + n +
CHCl3
0.05 %V/V
CHCL3
0.75% 0.075%V/V
MW 5min
@800W +
pH4
MW + HCl +
G + n
5 min @ 800W
+ addition of
HCL 25% for
pH4 during 17hr
CHCL3
0.10% 0.10%V/V
MW
3min@800W
+ pH4
MW + HCl +
G + n
3 min @ 800W
+ addition of
HCL 25% for
pH4 during 17hr
CHCL3
0.15% 0.15%V/V
CHCL3
0.075 + pH4
G + n +
CHCl3
0.075 %V/V +
addition of
HCL 25% for
pH4 during 17hr
Fe III
(5mM)
G + n + Fe
III
5 mM
Fe III (7mM)
+ pH4
G + n + Fe III
7mM +pH4
(17hr) CONTROL
SAMPLES
Blanc
glucose
Blanc
Glucose
G+n+ no pretreatment
Fe III (7mM)
+ pH6
7mM+pH6
(17hr)
Blanc no
glucose
Blanc no
Glucose
No G+ no pretreatment
G: Glucose; n: nutrients; WB: Water Bath; MW: Microwaves; CHCL3: Chloroform; Fe III: Iron 3 Chloride; HCl: Hydrochloric
acid; BES: Bromo-ethane-sulfonate;