McBryde, Gardner, de Barros Lopes, and Jiranek (2006) used
sequential batch fermentation for adaptive evolution of a wine
yeast strain. While the original strain gave rise to sluggish fermentations
in a 200 g/L sugars synthetic must, the final mixed
culture obtained from adaptive evolution was able to complete
total sugar consumption. Cadière, Ortiz-Julien, Camarasa, and
Dequin (2011) using sequential batch cultivation on gluconate as
carbon source of a wine commercial strain obtained evolved strains
with increased flux towards the pentose phosphate pathway.
In this work we have used adaptive laboratory evolution in
continuous culture, with ethanol as the challenging agent, in order to
obtain derivatives of two industrial wine yeast strains. Some of these
evolved strains show improved fermentation kinetics under harsh
environmental conditions, among other phenotypic modifications.
McBryde, Gardner, de Barros Lopes, and Jiranek (2006) used
sequential batch fermentation for adaptive evolution of a wine
yeast strain. While the original strain gave rise to sluggish fermentations
in a 200 g/L sugars synthetic must, the final mixed
culture obtained from adaptive evolution was able to complete
total sugar consumption. Cadière, Ortiz-Julien, Camarasa, and
Dequin (2011) using sequential batch cultivation on gluconate as
carbon source of a wine commercial strain obtained evolved strains
with increased flux towards the pentose phosphate pathway.
In this work we have used adaptive laboratory evolution in
continuous culture, with ethanol as the challenging agent, in order to
obtain derivatives of two industrial wine yeast strains. Some of these
evolved strains show improved fermentation kinetics under harsh
environmental conditions, among other phenotypic modifications.
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