Culture media
Semi solid MS medium (Murashige and Skoog, 1962) containing 3% sucrose with varying concentration of phytohormones was employed for callus formation and roots and shoot regenerations. Combination of auxin (IAA, IBA, NAA, 2, 4-D) was also employed for root and shoot regeneration. Coconut milk with a high level of sugar and other salts were also used as growth supplements in plant tissue culture.