as a functional food since prehistoric times. They are one of the less explored sources of pharmacological candidates,
and few previous studies had found anti-diabetic activities in various marine algae[9].Caulerpa lentillifera (C. lentillifera) is native to tropical areas of the Indian and Pacific Oceans, but it is currently discovered in the coast of Korea due to abnormal temperature. C. lentillifera is a popular edible species high in minerals, dietary fibers, vitamin A, vitamin C, and several essential unsaturated fatty acids, which is eaten fresh or salted for later use[10]. Recently, anti-cancer[11], antioxidative[12], and lipid-lowering[13] activities of C. lentillifera extract have been documented. Also, C. lentillifera has
been used traditionally in the Philippines for the treatment of diabetes. However, there are no experimental evidences. Therefore, our hypothesis is to investigate whether C.lentillifera extract increases insulin secretion and glucose uptake in pancreatic-β cells and adipocytes, respectively. To test the hypothesis, we measured the effect of C.lentillifera extract on DPP-IV and α-glucosidase enzymes using in vitro enzyme assay. Subsequently, we measured insulin secretion and glucose uptake in rat insulinoma (RIN) cells and 3T3-L1 adipocytes, respectively.