KITS:
Kits suitable for performing 50 assays are available from Megazyme. The
kits contain the full assay method plus:
Bottle 1: Buffer (25 mL, pH 5.5) and sodium azide (0.02% w/v)
as a preservative.
Stable for > 2 years at 4°C.
Bottle 2: Phytase suspension (1.2 mL).
Stable for > 2 years at 4°C.
Bottle 3: Buffer (25 mL, pH 10.4), plus MgCl2, ZnSO4 and
sodium azide (0.02% w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 4: Alkaline phosphatase suspension (1.2 mL).
Stable for > 2 years at 4°C.
Bottle 5: Phosphorus standard solution (24 mL, 50 µg/mL)
and sodium azide (0.02% w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 6: Oat flour control powder (5 g; Phosphorus content,
see bottle).
Stable for > 5 years at room temperature.
PREPARATION OF REAGENT SOLUTIONS/SUSPENSIONS:
1. Use the contents of bottle 1 as supplied.
Stable for > 2 years at 4°C.
2. Use the contents of bottle 2 as supplied. Before opening for
the first time, shake the bottles to remove any enzyme that
may have settled on the rubber stopper. Subsequently store
the bottles in an upright position. Swirl the bottle to mix
contents before use.
Stable for > 2 years at 4°C.
3. Use the contents of bottle 3 as supplied.
Stable for > 2 years at 4°C.
4. Use the contents of bottle 4 as supplied. Before opening for
the first time, shake the bottle to remove any enzyme that
may have settled on the rubber stopper. Subsequently store
the bottle in an upright position. Swirl the bottle to mix
contents before use.
Stable for > 2 years at 4°C.
5. Use the contents of bottle 5 as supplied.
Stable for > 2 years at 4°C
Series: Suitable for 50 assays kit from Megazyme the full test consists of a method plus: Bottle 1: buffer (25 mL, pH 5.5) and sodium azide (0.02% w / v) as a preservative stable> 2 years. 4 ° C Bottle 2: Properties suspension (1.2 ml) enclosure> 2 years at 4 ° C Bottle 3: buffer (25 mL, pH 10.4), and MgCl2, ZnSO4 and sodium azide (0.02% w / v) is. preservative stable> 2 years at 4 ° C Bottle 4: Alkaline Phosphatase sonatas suspension (1.2 ml) enclosure> 2 years at 4 ° C bottle 5: Phosphorus standard solution (24 mL micrograms is 50. mL) and sodium azide (0.02% w / v) as a preservative stable> 2 years at 4 ° C Bottle 6: oatmeal powder control (5 g phosphorus content bottles) enclosure> 5 years at room temperature to prepare treatments. Reagents solutions / services: 1. The flesh of one bottle provides stable> 2 years at 4 ° C for two. The flesh of the two subsequent bottle before opening time. Shake well to remove any enzyme that may have been in the rubber store later the bottle in an upright position , rotate the bottle to mix the contents before using the pen> 2 years at 4 ° C 3. Three bottles of beef held stable> 2 years at 4 ° C for 4. The meat of four bottles arranged before the opening of the first. Shake well to remove any enzyme that may have been in the rubber store later the bottle in an upright position , rotate the bottle to mix the contents before using the pen> 2 years at 4 ° C for 5. 5 bottles of beef held stable> 2 years at 4 ° C.
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