Collection of blood and serum
To quantify expression of IFN-c, whole blood samples
were collected with anti coagulant (10 IU of heparin per ml
of blood) at different interval from pre-immunized and
post-immunized calves under sterile condition. Separate
blood samples were also collected without anti-coagulant
for separation of serum. The collection schedule was day 0
(prior to primary immunization), 21st day, 45th day, 75th
day, 100th day and 140th day post immunization. The
serum samples were stored at -80 C for analysis of
humoral immune responses.