Hence, DNA fragment migrate via anelectrical field and form the tail of a comet-like-shaped fluores-cence signal while the yield of unrepaired DNA breaks increasesthe size of the head of the comet. Unfortunately, a real confusionappeared in literature between comet assays performed in alkalinesolution that concerns SSB and DSB (denatured as SSB), whereasneutral comet, more difficult to develop, reflects DSB response only