The use of M gene based two-step SYBR Green I real time RT-PCR for molecular diagnosis of PPR has not been reported so far. The availability of such technique will provide an alternative to the N gene based real-time RT-PCR diagnostic assays that already exist (especially in terms of its sen-sitivity); thereby helping in rapid clinical diagnosis of PPR. In thisregard, the present study developed a two-step SYBR Green I based real time RT-PCR assay for the detection and quantitation of PPRV in clinical samples.