2.3.3. Preparation of whole wheat flour by pretreated bran recombining
In these processes, whole wheat flours were prepared by recombining stabilized or pretreated bran back to white flour according to their original proportions.
For extrudes bran recombining (EBR) process, the bran fraction was passed through a 80 mesh sieve and tempered to 40 ± 2 g/100 g moisture content, stirred, evenly blended and rest
for 1 h. 
A co-rotating double screw extruder from Saixin (Ji'nan Saixin Machinery Co., Ltd., Shandong, CN) was used. The screw configuration was composed of conveying and mixing elements
with reverse elements in the last section of the barrel.
The extruder was operated at a feed rate of 25e30 kg h1, screw speed 120 r/min, mass temperature 150 C, The extruded samples were collected and dried slowly in an oven at 60 C for 16 h. After
ground by a Perten LABORATORY MILL 3100, the collected bran fraction was passed through a CQ20 screen and evenly blended with white flour according to its original proportions to get
whole wheat flour.