where D1 and D2 are the diameters of the smaller
and the larger columns, respectively, and L1 and
L2 are the respective lengths. We used L1=L2=
15 cm and then f and m were equal to 13. Thus,
using a new column (15.0×9.0 cm) the mobile
phase flow rate was 1300 ml h−1 (0.34 ml cm−2
min−1) and the loading of the phycobiliproteins
mixture was 95 mg. Operating in these conditions
(Fig. 5) the same two fractions were separated as
at the small scale.
Although the most utilized criteria to check
biliprotein solutions purity is the absorbance ratio
Amax visible/A280, the purity must be followed by
Fig. 3. Influence of loading in the recovery of phycobiliproteins
at small