The use of mass spectrometry for the analysis of proteins and peptides is reviewed. Sample preparation and ionization methods are discussed. The use of coupled liquid chromatography is described. The approach is exemplified with a number of applications including quality control of synthetic and recombinant materials. The approaches for addressing post-translational change such as glycosylation are also given.
Keywords
ESI, Genetic mutations; Glycosylation; Hydrogen-deuterium exchange; LC-MS; MALDI; Peptide sequencing; Phosphorylation; Proteomics; Recombinant proteins; Synthetic peptides; Trypsin digest
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This article is reproduced from the previous edition, Copyright 1999, Elsevier Ltd.